1M Sulphuric Acid Solution Preparation
Introduction
Sulphuric acid (H2SO4) is a strong acid widely used in various laboratory applications, including analytical chemistry, biology, and biochemistry. In many cases, a 1M sulphuric acid solution is required for specific experiments or procedures. In this article, we will outline the step-by-step preparation of a 1M sulphuric acid solution.
Materials
- Sulphuric acid (H2SO4) - concentrated (98%)
- Distilled water
- Volumetric flask (1 liter)
- Pipette or measuring cylinder
- Gloves and safety goggles (optional)
Preparation
Step 1: Calculate the Volume of Sulphuric Acid Required
To prepare a 1M sulphuric acid solution, you need to calculate the volume of concentrated sulphuric acid required. The molarity of the solution (1M) is defined as the number of moles of solute (H2SO4) per liter of solution. The molecular weight of sulphuric acid is 98.08 g/mol.
For a 1M solution, you need:
- 1 mole of sulphuric acid (H2SO4) = 98.08 g
- Density of concentrated sulphuric acid = approximately 1.84 g/mL
Therefore, the volume of concentrated sulphuric acid required is:
- 98.08 g / 1.84 g/mL = 53.3 mL
Step 2: Measure the Sulphuric Acid
Using a pipette or measuring cylinder, carefully measure 53.3 mL of concentrated sulphuric acid into the volumetric flask.
Step 3: Add Distilled Water
Slowly add distilled water to the volumetric flask while stirring until the total volume reaches 1 liter. Make sure to add the water slowly to prevent splashing or boiling of the acid.
Step 4: Mix and Label
Mix the solution well and label the flask with the concentration (1M), date, and your name.
Safety Precautions
- Wear gloves and safety goggles when handling sulphuric acid to prevent skin and eye irritation.
- Handle the acid in a well-ventilated area to prevent inhalation of fumes.
- Avoid splashing or spilling the acid, as it can cause burns and damage surfaces.
Conclusion
By following these steps, you should now have a 1M sulphuric acid solution prepared for your laboratory applications. Remember to handle the acid with care and follow proper safety protocols to avoid accidents.
