0.1 M Sodium Phosphate Buffer pH 8
Introduction
Sodium phosphate buffer is a commonly used buffering agent in various biochemical and molecular biological applications. A 0.1 M sodium phosphate buffer with a pH of 8 is a specific formulation of this buffer that is widely used in many laboratory procedures.
Composition
A 0.1 M sodium phosphate buffer pH 8 is composed of:
- 0.1 M Sodium phosphate monobasic (NaH2PO4)
- 0.1 M Sodium phosphate dibasic (Na2HPO4)
The pH of the buffer is adjusted to 8 by mixing the monobasic and dibasic forms of sodium phosphate in the appropriate ratio.
Preparation
To prepare 1 liter of 0.1 M sodium phosphate buffer pH 8, follow these steps:
Step 1: Prepare the Stock Solutions
- Dissolve 13.8 g of NaH2PO4 in 1 liter of water to prepare the monobasic sodium phosphate stock solution (0.1 M).
- Dissolve 13.8 g of Na2HPO4 in 1 liter of water to prepare the dibasic sodium phosphate stock solution (0.1 M).
Step 2: Mix the Stock Solutions
- Mix 350 mL of the monobasic sodium phosphate stock solution with 650 mL of the dibasic sodium phosphate stock solution to prepare the 0.1 M sodium phosphate buffer pH 8.
Properties
The 0.1 M sodium phosphate buffer pH 8 has the following properties:
- pH: 8
- Molarity: 0.1 M
- Concentration: 1.38 g/100 mL
- Temperature: 25°C
Applications
The 0.1 M sodium phosphate buffer pH 8 is widely used in various applications, including:
- Molecular biology: As a buffering agent in PCR reactions, DNA sequencing, and protein expression experiments.
- Biochemistry: As a buffering agent in enzyme assays, protein purification, and chromatography.
- Pharmaceuticals: As a buffering agent in pharmaceutical formulations.
Advantages
The 0.1 M sodium phosphate buffer pH 8 has several advantages, including:
- Stability: The buffer is stable over a wide range of temperatures and pH values.
- Compatibility: The buffer is compatible with many biological molecules, including proteins, DNA, and RNA.
- Flexibility: The buffer can be used in a variety of applications, including molecular biology, biochemistry, and pharmaceuticals.
In conclusion, the 0.1 M sodium phosphate buffer pH 8 is a versatile and widely used buffering agent in many laboratory applications. Its stability, compatibility, and flexibility make it an ideal choice for many researchers and scientists.
