0.1 M Sodium Edetate Preparation
Sodium edetate, also known as EDTA (ethylenediaminetetraacetic acid), is a widely used chelating agent in various fields such as analytical chemistry, biochemistry, and pharmacology. In this article, we will outline the preparation of 0.1 M sodium edetate solution, which is commonly used in laboratory settings.
Materials Required
- Sodium edetate (EDTA) dihydrate (C10H14N2O8Na2.2H2O)
- Distilled water
- pH paper or pH meter
Preparation of 0.1 M Sodium Edetate Solution
Step 1: Weighing and Dissolving EDTA
Weigh: Weigh accurately 3.72 grams of sodium edetate dihydrate (C10H14N2O8Na2.2H2O) in a weighing boat or a small cup.
Dissolve: Transfer the weighed EDTA into a 100 mL beaker or a glass container. Add about 50 mL of distilled water to the beaker and stir gently until the EDTA is completely dissolved.
Step 2: Adjusting pH
pH Adjustment: Use pH paper or a pH meter to check the pH of the solution. The ideal pH range for sodium edetate solution is between 6.0 and 7.0. If the pH is higher or lower than this range, adjust it by adding a small amount of 1 M sodium hydroxide (NaOH) or 1 M hydrochloric acid (HCl) solution.
Step 3: Dilution and Filtration
Dilution: Once the pH is adjusted, add more distilled water to the solution to make a total volume of 100 mL.
Filtration: Filter the solution through a filter paper or a membrane filter (0.2 μm) to remove any impurities.
Storage: Store the 0.1 M sodium edetate solution in a clean, sterilized bottle or container, and keep it in a cool, dry place.
Safety Precautions
- Handle the EDTA powder and solution with gloves and safety glasses to avoid skin contact and eye irritation.
- Store the solution in a well-ventilated area, away from children and pets.
Conclusion
The 0.1 M sodium edetate solution is now ready to use in various laboratory applications, such as chelation studies, metal analysis, and buffer solutions. Remember to follow proper safety protocols and handle the solution with care to ensure accurate and reliable results.
