0.1 M Sodium Carbonate Buffer pH 9
Introduction
Sodium carbonate buffer is a widely used buffer solution in various biochemical and biological applications. It is commonly used to maintain a specific pH range in solutions, particularly in alkaline conditions. In this article, we will discuss the preparation and characteristics of a 0.1 M sodium carbonate buffer at pH 9.
Preparation of 0.1 M Sodium Carbonate Buffer pH 9
To prepare a 0.1 M sodium carbonate buffer at pH 9, you will need the following reagents:
- Sodium carbonate (Na2CO3): 10.6 g
- Sodium bicarbonate (NaHCO3): 8.4 g
- Distilled water: 1 L
Instructions:
- Weigh out 10.6 g of sodium carbonate (Na2CO3) and 8.4 g of sodium bicarbonate (NaHCO3) and transfer them to a 1 L beaker.
- Add about 900 mL of distilled water to the beaker and stir until the salts are completely dissolved.
- Adjust the pH of the solution to 9.0 using a pH meter and hydrochloric acid (HCl) or sodium hydroxide (NaOH) as needed.
- Once the pH is adjusted, add more distilled water to bring the final volume to 1 L.
- Mix the solution well and store it in a clean glass bottle at room temperature.
Characteristics of 0.1 M Sodium Carbonate Buffer pH 9
The 0.1 M sodium carbonate buffer at pH 9 has the following characteristics:
- pH: 9.0 ± 0.1
- Molarity: 0.1 M
- Buffer capacity: High
- Stability: Stable at room temperature for several months
- Interference: None known
Applications of 0.1 M Sodium Carbonate Buffer pH 9
The 0.1 M sodium carbonate buffer at pH 9 has numerous applications in various fields, including:
- Biochemistry: Used as a buffer in enzyme assays, protein purification, and other biochemical reactions.
- Molecular biology: Used in PCR reactions, DNA sequencing, and other molecular biology applications.
- Pharmaceuticals: Used as a buffer in pharmaceutical formulations and as a stabilizer in vaccines.
- Food industry: Used as a buffer in food processing and as a stabilizer in food products.
Conclusion
In conclusion, the 0.1 M sodium carbonate buffer at pH 9 is a widely used buffer solution in various biochemical and biological applications. Its high buffer capacity and stability make it an ideal choice for many applications. By following the preparation instructions outlined above, you can easily prepare this buffer solution in your laboratory.
