0.1 M Sodium Carbonate-Bicarbonate Buffer pH 9.0
Introduction
Sodium carbonate-bicarbonate buffer is a commonly used buffer solution in biochemical reactions, especially in protein assays and enzymatic reactions. The buffer solution is a mixture of sodium carbonate (Na2CO3) and sodium bicarbonate (NaHCO3) that provides a stable pH environment for enzymes and proteins to function optimally. In this article, we will discuss the preparation and characteristics of 0.1 M sodium carbonate-bicarbonate buffer pH 9.0.
Preparation
To prepare 0.1 M sodium carbonate-bicarbonate buffer pH 9.0, you will need the following chemicals:
- Sodium carbonate (Na2CO3)
- Sodium bicarbonate (NaHCO3)
- Distilled water
The preparation of the buffer solution involves dissolving the required amounts of sodium carbonate and sodium bicarbonate in distilled water. The amounts of each chemical can be calculated using the following formula:
Formula:
Na2CO3: 1.06 g
NaHCO3: 8.40 g
Distilled water: 1 L
Dissolve 1.06 g of sodium carbonate and 8.40 g of sodium bicarbonate in 1 L of distilled water. Mix the solution well until the chemicals are completely dissolved.
Characteristics
The 0.1 M sodium carbonate-bicarbonate buffer pH 9.0 has the following characteristics:
- pH: 9.0
- Concentration: 0.1 M
- Buffer capacity: High
- Temperature range: 20-25°C
- Stability: Stable for 6 months at 4°C
Applications
The 0.1 M sodium carbonate-bicarbonate buffer pH 9.0 has a wide range of applications in biochemical reactions, including:
- Enzyme assays: The buffer solution provides a stable pH environment for enzymes to function optimally.
- Protein purification: The buffer solution is used to purify proteins and prevent protein denaturation.
- Cell culture: The buffer solution is used to maintain a stable pH environment for cell growth and development.
Conclusion
In conclusion, the 0.1 M sodium carbonate-bicarbonate buffer pH 9.0 is a commonly used buffer solution in biochemical reactions. The buffer solution provides a stable pH environment for enzymes and proteins to function optimally. Its high buffer capacity and stability make it an ideal choice for various biochemical applications.
